What Is Phenol Red?
Phenol red, or phenolsulfonephthalein, is a dark to red odorless crystalline powder used as a pH indicator and for various other applications. It changes color from yellow in neutral solutions to red in alkaline conditions, making it useful for chemical experiments, biochemical analysis, and cell culture monitoring. Synthesized by reacting phenol with o-sulfobenzoic anhydride, phenol red’s versatility extends to kidney function tests due to its complete excretion in urine.
Uses of Phenol Red
As a pH indicator, phenol red aids in determining the acidity or alkalinity of solutions. In cell culture, its color change helps monitor the health and conditions of cells. Additionally, it’s utilized in kidney function tests, exploiting its excretion through urine to assess renal health.
Characteristics of Phenol Red
Phenol red has a molecular weight of 354.38 and exhibits properties such as decomposing above 300°C and being nearly insoluble in water. It’s slightly soluble in ethanol and soluble in dilute sodium hydroxide solution. The compound is stable under normal conditions but sensitive to light and strong oxidizing agents.
Other Information on Phenol Red
1. Safety of Phenol Red
It’s classified under GHS for skin and eye irritation risks. Precautions include using protective gear during handling and ensuring proper ventilation in work areas. Despite its classification, no significant toxicity or environmental hazard has been confirmed.
2. Handling and Storage
Handling should involve protective equipment and avoiding ingestion, while storage requires sealed containers in cool, dark places away from incompatible materials. Compliance with relevant laws and regulations is essential for disposal.